"Al-Kofahi O, Radke RJ, Goderie SK, Shen Q, Temple S, Roysam B: Automated cell lineage construction: a rapid method to analyze clonal development established with murine neural progenitor cells. Cell Cycle 2006, 5:327–35." Motility & Counting Thresholding & Watershed Cell Unknown Unknown Phase contrast & Wide-field fluorescence X-Y-T "Bahnson A, Athanassiou C, Koebler D, Qian L, Shun T, Shields D, Yu H, Wang H, Goff J, Cheng T, Houck R, Cowsert L: Automated measurement of cell motility and proliferation. BMC Cell Biol 2005, 6:19." Motility & Counting Region growing & Active contours + Level Set Cell Object-level evaluation Unknown Bright-field X-Y-T "Bai W, Zhou X, Zhu J, Ji L, Wong STC: Tracking of migrating Glioma cells in feature space. In 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro. IEEE; 2007:272–275." Motility Thresholding Cell Unknown Single core CPU Bright-field & Wide-field fluorescence X-Y-T "Bajcsy P, Lee S-C, Lin a, Folberg R: Three-dimensional volume reconstruction of extracellular matrix proteins in uveal melanoma from fluorescent confocal laser scanning microscope images. J Microsc 2006, 221(Pt 1):30–45." Location Thresholding Other Object-level evaluation Unknown Confocal fluorescence X-Y-Z "Bise R, Li K, Eom S: Reliably tracking partially overlapping neural stem cells in dic microscopy image sequences. In MICCAI Workshop on Optical Tissue Image analysis in Microscopy, Histopathology and Endoscopy. Imperial College London; 2009:67–77." Motility Thresholding Cell Object-level evaluation Unknown Differential interference contrast X-Y-T "Buggenthin F, Marr C, Schwarzfischer M, Hoppe PS, Hilsenbeck O, Schroeder T, Theis FJ: An automatic method for robust and fast cell detection in bright field images from high-throughput microscopy. BMC Bioinformatics 2013, 14:297." Counting Other Cell Object-level evaluation Cluster Bright-field X-Y-T "Celli JP, Rizvi I, Evans CL, Abu-Yousif AO, Hasan T: Quantitative imaging reveals heterogeneous growth dynamics and treatment-dependent residual tumor distributions in a three-dimensional ovarian cancer model. J Biomed Opt 2010, 15:051603." Geometry Thresholding Other Visual inspection Unknown Dark-field & Phase contrast X-Y-Z & X-Y-Z-T "Chalfoun J, Kociolek M, Dima A, Halter M, Cardone A, Peskin A, Bajcsy P, Brady M: Segmenting time-lapse phase contrast images of adjacent NIH 3T3 cells. J. Microsc. 2013, 249:41–52." Geometry Other Cell Pixel-level evaluation Unknown Phase contrast X-Y-T "Chalfoun J, Majurski M, Dima A, Stuelten C, Peskin A: FogBank?: A Single Cell Segmentation across Multiple Cell Lines and Image Modalities. BMC Bioinformatics 2014, 15:431." Geometry Watershed & Other Cell Pixel-level evaluation & Visual inspection Unknown Phase contrast & Bright-field & Wide-field fluorescence X-Y-T "Chalfoun J, Majurski M, Peskin A, Breen C, Bajcsy P: Empirical Gradient Threshold Technique for Automated Segmentation across Image Modalities and Cell Lines. J. Microsc. 2014:1–18." Geometry Thresholding Cell Pixel-level evaluation & Visual inspection Unknown Phase contrast & Differential interference contrast & Bright-field & Wide-field fluorescence X-Y-T "Chowdhury S, Ruusuvuori P, Liberali P: Automated cell tracking and cell lineage construction with improved performance. In Proceedings of the Sixth International Workshop on Computational Systems Biology (WCSB). Edited by Manninen T et al. Aarhus, Denmark; 2009:2–5." Motility Active contours + Level Set Nucleus Object-level evaluation Unknown Wide-field fluorescence X-Y-T "Cohen AR, Gomes FL a F, Roysam B, Cayouette M: Computational prediction of neural progenitor cell fates. Nat Methods 2010, 7:213–8." Geometry Other Cell Unknown Multi-core CPU Phase contrast X-Y-T "Debeir O, Camby I, Kiss R, Van Ham P, Decaestecker C: A model-based approach for automated in vitro cell tracking and chemotaxis analyses. Cytometry A 2004, 60:29–40." Motility Active contours + Level Set Cell & Synthetic (digital model) Visual inspection Single core CPU Phase contrast X-Y-T "Delgado-Gonzalo R, Chenouard N, Unser M: Spline-based deforming ellipsoids for interactive 3D bioimage segmentation. IEEE Trans Image Process 2013, 22:3926–3940." Geometry Active contours + Level Set Cell & Other & Synthetic (digital model) Pixel-level evaluation & Visual inspection Single core CPU Confocal fluorescence X-Y-Z "Du C-J, Hawkins PT, Stephens LR, Bretschneider T: 3D time series analysis of cell shape using Laplacian approaches. BMC Bioinformatics 2013, 14:296." Geometry & Location Active contours + Level Set & Graph-based Cell & Synthetic (digital model) Pixel-level evaluation & Visual inspection Single core CPU Confocal fluorescence X-Y-Z-T "Dzyubachyk O, Niessen W, Meijering E: Advanced Level-Set Based Multiple-Cell Segmentation and Tracking In Time-Lapse Fluorescence Microscopy Images. In Proceedings of the 5th IEEE Int. Symp. IEEE In Biomed. Imaging From Nano to Macro. IEEE Computer Society; 2008:185–188." Motility Active contours + Level Set Nucleus Visual inspection Unknown Confocal fluorescence X-Y-Z-T "Dzyubachyk O, van Cappellen W a., Essers J, Niessen W, Meijering E: Energy minimization methods for cell motion correction and intracellular analysis in live-cell fluorescence microscopy. In 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro. Ieee; 2009:1127–1130." Motility Active contours + Level Set Nucleus Unknown Unknown Confocal fluorescence X-Y-Z-T "Dzyubachyk O, Van Cappellen WA, Essers J, Niessen WJ, Meijering E: Advanced level-set-based cell tracking in time-lapse fluorescence microscopy. IEEE Trans Med Imaging 2010, 29:852–867." Motility Active contours + Level Set Cell Pixel-level evaluation Unknown Confocal fluorescence X-Y-T "Eck S, Rohr K, Biesdorf A, Katharina M-O, Rippe K, Stefan W: A 3D Intensity Model Based on Spherical Harmonics For Automatic 3D Segmentation of Heterochromatin Foci. In 2013 IEEE 10th International Symposium on Biomedical Imaging: From Nano to Macro. San Francisco; 2013:1484–1487." Location Partial Derivative Equations (PDE) Other Pixel-level evaluation Unknown Confocal fluorescence X-Y-Z "Fusco L, Benmansour F, Fua P, Pertz O, Smith K: Automated Quantification of Morphodynamics for High-Throughput Live Cell Time-Lapse Datasets. In IEEE 10th International Symposium on Biomedical Imaging (ISBI). San Francisco, CA: IEEE; 2013:664–667." Geometry Thresholding & Region growing & Graph-based Cell Unknown Single core CPU Wide-field fluorescence X-Y-T "Hadjidemetriou S, Gabrielli B, Mele K, Vallotton P: Detection and tracking of cell divisions in phase contrast video microscopy. In Proc. of the Third MICCAI Worshop on Microscopic Image Analysis with Applications in Biology. New York, NY, USA: MICCAI; 2008." Motility & Counting Other Cell Visual inspection Single core CPU Phase contrast X-Y-T "Hand AJ, Sun T, Barber DC, Hose DR, MacNeil S: Automated tracking of migrating cells in phase-contrast video microscopy sequences using image registration. J Microsc 2009, 234:62–79." Motility Watershed Cell Object-level evaluation Unknown Phase contrast & Wide-field fluorescence X-Y-T "Held M, Schmitz MH a, Fischer B, Walter T, Neumann B, Olma MH, Peter M, Ellenberg J, Gerlich DW: CellCognition: time-resolved phenotype annotation in high-throughput live cell imaging. Nat. Methods 2010, 7:747–54." Motility & Counting Thresholding & Watershed Cell Unknown Single core CPU Wide-field fluorescence X-Y-T "Herberich G, Windoffer R, Leube R, Aach T: 3D segmentation of keratin intermediate filaments in confocal laser scanning microscopy. In Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Boston, MA: IEEE EMBS; 2011:7751–4." Location Thresholding Synthetic (digital model) & Other Pixel-level evaluation & Visual inspection Unknown Confocal fluorescence X-Y-Z "Himeno-Ando A, Izumi Y, Yamaguchi A, Iimura T: Structural differences in the osteocyte network between the calvaria and long bone revealed by three-dimensional fluorescence morphometry, possibly reflecting distinct mechano-adaptations and sensitivities. Biochem Biophys Res Commun 2012, 417:765–70." Geometry Thresholding Cell Visual inspection Unknown Confocal fluorescence X-Y-Z "Hodneland E, Bukoreshtliev N V, Eichler TW, Tai X-C, Gurke S, Lundervold A, Gerdes H-H: A unified framework for automated 3-d segmentation of surface-stained living cells and a comprehensive segmentation evaluation. IEEE Trans Med Imaging 2009, 28:720–38." Location Watershed & Active contours + Level Set Cell Pixel-level evaluation Single core CPU Wide-field fluorescence & Confocal fluorescence X-Y-Z "Hu Y, Osuna-Highley E, Hua J, Nowicki TS, Stolz R, McKayle C, Murphy RF: Automated analysis of protein subcellular location in time series images. Bioinformatics 2010, 26:1630–6." Location Thresholding Cell Unknown Unknown Wide-field fluorescence & Confocal fluorescence X-Y-T & X-Y-Z & X-Y-Z-T "Hua J, Sima C, Cypert M, Gooden GC, Shack S, Alla L, Smith E a, Trent JM, Dougherty ER, Bittner ML: Tracking transcriptional activities with high-content epifluorescent imaging. J Biomed Opt 2012, 17:046008." Counting & Intensity Thresholding & Watershed Nucleus Object-level evaluation Single core CPU Wide-field fluorescence X-Y-T "Huh S, Eom S, Bise R, Yin Z, Kanade T: Mitosis detection for stem cell tracking in phase-contrast microscopy images. In Biomedical Imaging: From Nano to Macro, 2011 IEEE International Symposium on. IEEE; 2011:2121–2127." Motility Thresholding Cell Object-level evaluation Unknown Phase contrast X-Y-T "Huth J, Buchholz M, Kraus JM, Schmucker M, von Wichert G, Krndija D, Seufferlein T, Gress TM, Kestler H a: Significantly improved precision of cell migration analysis in time-lapse video microscopy through use of a fully automated tracking system. BMC Cell Biol 2010, 11:24." Motility Thresholding & Morphological Cell Object-level evaluation Single core CPU Differential interference contrast X-Y-T "Indhumathi C, Cai YY, Guan YQ, Opas M: An automatic segmentation algorithm for 3D cell cluster splitting using volumetric confocal images. J Microsc 2011, 243:60–76." Location Active contours + Level Set Nucleus & Synthetic (digital model) Object-level evaluation & Visual inspection Single core CPU Confocal fluorescence X-Y-Z "Juba D, Cardone A, Ip CY, Simon Jr. CG, Tison CK, Kumar G, Brady M, Varshney A: Parallel Geometric Classification of Stem Cells by Their 3D Morphology. Comput Sci Discov 2013, 6." Geometry Thresholding Cell Unknown GPU Confocal fluorescence X-Y-Z "Kachouie NN, Fieguth P, Ramunas J, Jervis E: A Statistical Thresholding Method for Cell Tracking. 2006 IEEE Int Inf Technol 2006:222–227." Motility Thresholding Cell Unknown Unknown Phase contrast X-Y-T "Kerschnitzki M, Kollmannsberger P, Burghammer M, Duda GN, Weinkamer R, Wagermaier W, Fratzl P: Architecture of the osteocyte network correlates with bone material quality. J Bone Miner Res 2013, 28:1837–45." Geometry Thresholding Cell Unknown Unknown Confocal fluorescence X-Y-Z "Kojic N, Huang A, Chung E, Tschumperlin D, So PTC: Quantification of three-dimensional dynamics of intercellular geometry under mechanical loading using a weighted directional adaptive-threshold method. Opt Express 2008, 16:12403." Geometry Thresholding Other Visual inspection Unknown Two-photon fluorescence X-Y-Z "Krausz E, de Hoogt R, Gustin E, Cornelissen F, Grand-Perret T, Janssen L, Vloemans N, Wuyts D, Frans S, Axel A, Peeters PJ, Hall B, Cik M: Translation of a Tumor Microenvironment Mimicking 3D Tumor Growth Co-culture Assay Platform to High-Content Screening. J Biomol Screen 2012." Geometry Thresholding Cell Unknown Multi-core CPU Confocal fluorescence X-Y-Z "Krzic U, Gunther S, Saunders TE, Streichan SJ, Hufnagel L: Multiview light-sheet microscope for rapid in toto imaging. Nat Methods 2012, 9:730–3." Motility & Location Watershed Nucleus & Cell Unknown Unknown Light sheet X-Y-Z-T "Law YN, Lee HK, Yip AM: Learning Dynamical Shape Prior for Level Set based Cell Tracking. Ann. Br. Mach. Vis. Assoc. 2013, 2013:1–14." Motility Active contours + Level Set Cell Pixel-level evaluation Unknown Wide-field fluorescence X-Y-T "Law YN: Cell tracking using phase-adaptive shape prior. J. Microsc. 2013, 252:149–58." Motility Active contours + Level Set Nucleus Pixel-level evaluation Unknown Wide-field fluorescence X-Y-T "Li K, Miller ED, Chen M, Kanade T, Weiss LE, Campbell PG: Cell population tracking and lineage construction with spatiotemporal context. Med Image Anal 2008, 12:546–66." Motility Active contours + Level Set Cell Object-level evaluation Single core CPU Phase contrast X-Y-T "Li K, Miller ED, Weiss LE: Online tracking of migrating and proliferating cells imaged with phase-contrast microscopy. In Proceedings of the 2006 Conference on Computer Vision and Pattern Recognition Workshop. IEEE Computer Society; 2006:8." Motility Thresholding Cell Unknown Unknown Phase contrast X-Y-T "Lin G, Adiga U, Olson K, Guzowski JF, Barnes C a, Roysam B: A hybrid 3D watershed algorithm incorporating gradient cues and object models for automatic segmentation of nuclei in confocal image stacks. Cytometry A 2003, 56:23–36." Location Watershed Nucleus Object-level evaluation Single core CPU Confocal fluorescence X-Y-Z "Lin G, Chawla MK, Olson K, Barnes CA, Guzowski JF, Bjornsson C, Shain W, Roysam B: A multi-model approach to simultaneous segmentation and classification of heterogeneous populations of cell nuclei in 3D confocal microscope images. Cytom Part A 2007, 71:724–736." Location Watershed Nucleus Object-level evaluation Unknown Confocal fluorescence X-Y-Z "Lou X, Kang M, Xenopoulos P, Muñoz-Descalzo S, Hadjantonakis A-K: A Rapid and Efficient 2D/3D Nuclear Segmentation Method for Analysis of Early Mouse Embryo and Stem Cell Image Data. Stem cell reports 2014, 2:382–97." Counting & Location Other Cell & Nucleus Object-level evaluation Multi-core CPU Confocal fluorescence & Differential interference contrast X-Y-T & X-Y-Z "Mange R, de Heras Ciechomski P, Swartz M: seeCell: Visualization and tracking dedicated to cell analysis. 2008 Int Conf Innov Inf Technol 2008:707–711." Motility Active contours + Level Set Cell Unknown Multi-core CPU Bright-field X-Y-T "McCullough DP, Gudla PR, Harris BS, Collins J a, Meaburn KJ, Nakaya M a, Yamaguchi TP, Misteli T, Lockett SJ: Segmentation of whole cells and cell nuclei from 3-D optical microscope images using dynamic programming. IEEE Trans Med Imaging 2008, 27:723–34." Location Active contours + Level Set Cell & Nucleus & Synthetic (digital model) Pixel-level evaluation & Visual inspection Single core CPU Confocal fluorescence X-Y-Z "Moeller M, Burger M, Dieterich P, Schwab A: A Framework for Automated Cell Tracking in Phase Contrast Microscopic Videos based on Normal Velocities. J Vis Commun Image Represent 2014, 25:396–409." Motility Active contours + Level Set Cell Pixel-level evaluation Unknown Phase contrast X-Y-T "Mosaliganti K, Cooper L: Reconstruction of cellular biological structures from optical microscopy data. IEEE Trans Vis Comput Graph 2008, 14:863–876." Location Active contours + Level Set Nucleus Pixel-level evaluation Single core CPU Confocal fluorescence X-Y-Z-T "Oh JH, Gertych A, Tajbakhsh J: Nuclear DNA Methylation and Chromatin Condensation Phenotypes Are Distinct Between Normally Proliferating / Aging , Rapidly Growing / Immortal , and Senescent Cells ABSTRACT :. Oncotarget 2013, 4:474–493." Location Thresholding Nucleus Unknown Unknown Confocal fluorescence X-Y-Z "Padfield D, Rittscher J, Roysam B: Spatio-temporal cell segmentation and tracking for automated screening. In Processings on the 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro; 2008:376–379." Motility Thresholding Cell Object-level evaluation Unknown Wide-field fluorescence X-Y-T "Padfield DR, Rittscher J, Sebastian T, Thomas N, Roysam B: Spatio-Temporal Cell Cycle Analysis Using 3D Level Set Segmentation of Unstained Nuclei in Line Scan Confocal Fluorescence Images. 3rd IEEE Int Symp Biomed Imaging Macro to Nano, 2006 :1036–1039." Motility Active contours + Level Set Nucleus Visual inspection Unknown Confocal fluorescence X-Y-T "Peng T, Murphy RF: Image-derived, three-dimensional generative models of cellular organization. Cytometry. A 2011, 79:383–91." Geometry & Location Thresholding Other Unknown Unknown Confocal fluorescence X-Y-Z "Pop S, Dufour A, Garrec J Le, Ragni C V, Buckingham ME, Meilhac SM, Olivo-Marin J-C: A Fast and Automated Framework for Extraction of Nuclei From Cluttered 3D Images in Fluorescence Microscopy. In 2011 IEEE International Symposium on Biomedical Imaging: From Nano to Macro; 2011(Ptr 335):2113–2116." Location Active contours + Level Set Nucleus Visual inspection Single core CPU Confocal fluorescence X-Y-Z "Russell RA, Adams NM, Stephens DA, Batty E, Jensen K, Freemont PS: Segmentation of fluorescence microscopy images for quantitative analysis of cell nuclear architecture. Biophys J 2009, 96:3379–89." Location Thresholding Nucleus & Synthetic (digital model) Pixel-level evaluation Single core CPU Confocal fluorescence X-Y-Z "Sbalzarini IF, Koumoutsakos P: Feature point tracking and trajectory analysis for video imaging in cell biology. J Struct Biol 2005, 151:182–95." Location Thresholding Synthetic (digital model) & Other & Synthetic (reference material) Unknown Unknown Wide-field fluorescence X-Y-T "Sigal A, Milo R, Cohen A, Geva-Zatorsky N, Klein Y, Alaluf I, Swerdlin N, Perzov N, Danon T, Liron Y, others: Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins. Nat Methods 2006, 3:525." Intensity Thresholding & Watershed Nucleus Unknown Unknown Wide-field fluorescence X-Y-T "Sigal A, Milo R, Cohen A, Geva-Zatorsky N, Klein Y, Liron Y, Rosenfeld N, Danon T, Perzov N, Alon U: Variability and memory of protein levels in human cells. Nature 2006, 444:643–6." Intensity Thresholding & Watershed Cell Unknown Unknown Wide-field fluorescence & Phase contrast X-Y-T "Singh S, Raman S, Rittscher J, Machiraju R: Segmentation Evaluation for Fluorescence Microscopy Images of Biological Objects. In MIAAB 2009 International Workshop Proceedings; 2009:1–5." Location Thresholding & Morphological Nucleus Pixel-level evaluation Unknown Confocal fluorescence X-Y-Z "Srinivasa G, Fickus M, Kova?evi? J: Active Contour-Based Multiresolution Transforms for the Segmentation of Fluorescence Microscope Images. In Proc. SPIE. edited by Van De Ville D, Goyal VK, Papadakis M2007:6701." Geometry Active contours + Level Set Cell Pixel-level evaluation Unknown Confocal fluorescence X-Y-Z "Srinivasa G, Fickus MC, Guo Y, Linstedt AD, Kovacevi? J: Active mask segmentation of fluorescence microscope images. IEEE Trans. Image Process. 2009, 18:1817–29." Geometry Active contours + Level Set Cell Pixel-level evaluation & Visual inspection Single core CPU Confocal fluorescence X-Y-Z "Takemoto S, Yokota H: Algorithm selection based on a region similarity metric for intracellular image segmentation. In Image Segmentation. Edited by Dr Ho P-G. InTech; 2011:419–434." Location Other Other Pixel-level evaluation Unknown Confocal fluorescence X-Y-Z "Wählby, C.; Sintorn, I.-M.; Erlandsson, F.; Borgefors, G.; Bengtsson, E.: Combining intensity , edge and shape information for 2D and 3D segmentation of cell nuclei in tissue sections. J Microsc 2004, 215(January):67–76." Location Watershed Nucleus Object-level evaluation & Visual inspection Single core CPU Wide-field fluorescence & Confocal fluorescence X-Y-Z "Wang M, Zhou X, Li F, Huckins J, King RW, Wong STC: Novel cell segmentation and online SVM for cell cycle phase identification in automated microscopy. Bioinformatics 2008, 24:94–101." Counting Watershed Nucleus Object-level evaluation Unknown Wide-field fluorescence X-Y-T "Wang Q, Niemi J, Tan C-M, You L, West M: Image segmentation and dynamic lineage analysis in single-cell fluorescence microscopy. Cytometry A 2010, 77:101–10." Motility & Counting Other Cell Unknown Unknown Phase contrast & Bright-field & Wide-field fluorescence X-Y-T "Winter M, Wait E, Roysam B, Goderie SK, Ali RAN, Kokovay E, Temple S, Cohen AR: Vertebrate neural stem cell segmentation, tracking and lineaging with validation and editing. Nat Protoc 2011, 6:1942–52." Counting Thresholding Cell Technique not specified Multi-core CPU Phase contrast X-Y-T "Xiong F, Ma W, Hiscock TW, Mosaliganti KR, Tentner AR, Brakke KA, Rannou N, Gelas A, Souhait L, Swinburne IA, Obholzer ND, Megason SG: Interplay of Cell Shape and Division Orientation Promotes Robust Morphogenesis of Developing Epithelia. Cell 2014, 159:415–427." Geometry Partial Derivative Equations (PDE) Cell Unknown Unknown Confocal fluorescence X-Y-Z-T "Yang X, Padfield D: Wavelet-initialized 3D level-set cell segmentation with local background support. In Biomedical Imaging (ISBI), 2014 IEEE 11th International Symposium on; 2014:814–817." Geometry Active contours + Level Set Cell & Synthetic (digital model) Pixel-level evaluation & Visual inspection Single core CPU Confocal fluorescence X-Y-Z "Yin Z, Kanade T, Chen M: Understanding the phase contrast optics to restore artifact-free microscopy images for segmentation. Med Image Anal 2012, 16:1047–62." Motility & Counting Thresholding Cell Pixel-level evaluation Unknown Phase contrast X-Y-T "Yin Z, Li K, Kanade T, Chen M: Understanding the Optics to Aid Microscopy Image Segmentation. In Medical Image Computing and Computer-Assisted Intervention--MICCAI 2010. Volume 13. Heidelberg: Springer; 2010:209–217." Motility & Counting Thresholding Cell Pixel-level evaluation Single core CPU Phase contrast X-Y-T "Yu W, Lee HK, Hariharan S, Bu W, Ahmed S: Evolving generalized Voronoi diagrams for accurate cellular image segmentation. Cytometry. A 2010, 77:379–86." Geometry Active contours + Level Set Cell Pixel-level evaluation Unknown Wide-field fluorescence X-Y-T "Zhou X, Li F, Yan J, Wong STC: A novel cell segmentation method and cell phase identification using Markov model. IEEE Trans Inf Technol Biomed 2009, 13:152–7." Location Watershed Nucleus Visual inspection Single core CPU Wide-field fluorescence X-Y-T "Zhu Y, Olson E, Subramanian A, Feiglin D, Varshney PK, Krol A: Neuronal nuclei localization in 3D using level set and watershed segmentation from laser scanning microscopy images. In Proc. SPIE. Volume 6914. Edited by Reinhardt JM, Pluim JPW.; 2008:691441–691441–8." Location Active contours + Level Set & Watershed Nucleus Object-level evaluation Unknown Confocal fluorescence X-Y-Z