GFP fluorescence was collected with a standard GFP filter cube set in the optical train. The CCD acquired images using 2x2 binning for 0.05 s (for phase contrast) and 0.3 s (for GFP fluorescence). All live cell data were acquired in the presence of phenol red free media. Three time-lapse image sets were generated, each containing 3 well-to-well replicates with roughly 238 time-lapse images at 520x696 pixels each.