2D Measurement of Spatial Distribution of Particles

Nanofibers
We imaged a specimen of aerosolized nanoparticles collected on a Si wafer using a FEI Helios NanoLab 650 (Hillsboro, OR) focused ion beam scanning electron microscope (FIB SEM). Small FOVs of a specimen were acquired and stored as TIFF images using the FEI’s MAPS correlative microscopy software. The small FOVs have an overlap of 10% in horizontal and vertical directions, and they form a grid of 40 x 59 raw image tiles. Each FOV image is 6.1MB (3072 x 2048 pixels, 8 bits per pixel) resulting in a total dataset size of 14 GB. Each pixel corresponds to 48.5 nanometers in horizontal and vertical dimensions.
Nanofibers in 3D Printing
We imaged debris from 3D printing process for two types of materials using a FEI Helios NanoLab 660 (Hillsboro, OR) focused ion beam scanning electron microscope (FIB SEM). A grid of field of views (FOVs) was acquired for each specimen in a Tiff file format with a spatial overlap of 10% in horizontal and vertical directions. Each FOV image is 6.1MB (3072 x 2048 pixels, 8 bits per pixel). The grid dimensions are 18 x 18 and 25 x 20 FOVs resulting in a total dataset size of 5 GB. Each pixel corresponds to approximately 65 nanometers in horizontal and vertical dimensions. These data sets provide information about spatial distribution and types of released particles.
Paper Samples
Another similar set of test images was acquired by imaging nanoscale dye particles on printing paper by the same FIB SEM microscope. The small FOVs have an overlap of 10% in horizontal and vertical directions, and they form a grid of 20 x 20 raw image tiles. Given these two data sets, materials scientists can characterize spatial distribution of nanoparticles.