Classification of 72 publications according to the classification criteria described below. The image segmentation-related publications are at the intersection of optical microscopy-based cellular measurements and automated segmentation.
The first order (frequency of occurrence) and second order (co-occurrence) statistics derived from classifications of the surveyed literature consist of three layers of information:
Access to 1st and 2nd Order Stats | Object of Interest | Imaging Modality | Data Axes | Segmentation | Segmentation Evaluation | Segmentation Acceleration Platform | Object Measurement |
Object of Interest | Click | Click | Click | Click | Click | Click | |
Imaging Modality | Click | Click | Click | Click | Click | ||
Data Axes | Click | Click | Click | Click | |||
Segmentation | Click | Click | Click | ||||
Segmentation Evaluation | Click | Click | |||||
Segmentation Acceleration Platform | Click | ||||||
Object Measurement |
Object of Interest |
Imaging Modality |
Data Axes |
Segmentation |
Segmentation Evaluation |
Segmentation Acceleration Platform |
Object Measurement |
Cell |
Phase Contrast |
X-Y-T |
Thresholding |
Visual inspection |
Unknown |
Motility |
Nucleus |
Differential Interference Contrast |
X-Y-Z |
Region Growing |
Unknown |
Single-core Central Processing Unit (CPU) |
Counting |
Synthetic (digital model) |
Bright-field |
X-Y-Z-T |
Morphological |
Technique is not specified |
Multi-core CPU |
Geometry |
Synthetic (reference material) |
Dark-field |
|
Watershed |
Object-level evaluation |
Graphics Processing Unit (GPU) |
Location |
Other (proteins, fibers, extracellular space, etc.) |
Confocal fluorescence |
|
Graph-based |
Pixel-level evaluation |
Cluster |
Intensity |
Wide-field fluorescence |
Active contours & Level Set |
|||||
Two-photon fluorescence |
Partial Derivative Equations (PDE) |
|||||
Light sheet |
Other |
Authors, "Survey Statistics of Automated Segmentations Applied to Optical Imaging of Mammalian Cells", BMC Bioinformatics, 2015, 16:330. doi: 10.1186/s12859-015-0762-2 (pdf)
Peter Bajcsy
peter.bajcsy@nist.gov
Phone: 301.975.2958